Bsd from aspergillus terreus or bsr from bacillus cereus. Blasticidin s hcl powder cas 3539 antibiotic ag scientific. The residue level of blasticidins in rice was below 0. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Blasticidin s inhibits translation by trapping deformed trna on the ribosome. Blasticidin s hcl stock solution mammalian 210 mgml. Cas 2079007 blasticidin s hydrochloride, 150477 blasticidin s hydrochloride msds toxicity property. Product name blasticidine s hydrochloride product number fb33855 synonyms. Due to high demand for rpi blasticidin s hydrochloride solution, 100mg liquid, availability is subject to change without notice. Blasticidin s inhibits protein synthesis and is active against bacteria, fungi, nematodes, and tumor cells. Also, blasticidin s inhibited ribonuclease p activity from dictyostelium discoideum and inhibited trna maturation in a dose dependent way 2. Safety data sheet according to 29cfr19101200 and ghs rev. Chemical and company identification bioshop canada inc. Material safety data sheet of blasticidin s hydrochloride.
To view your gsa or va contract pricing, log in using your account number, or become a registered user by contacting one of. You can also view your contract price by searching for this item s on gsa advantage. On thawing, use what you need and discard the unused portion. Material may be irritating to the mucous membranes and upper respiratory tract. Blasticidin s is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells.
The following data is based on the product molecular weight 458. Blasticidin is an effective selective agent for transformed cells. Bp2647100 synonyms no information available recommended use laboratory chemicals. Msds blasticidin s hydrochloride, 150477 blasticidin s. In mouse leukemia l5178y cells, blasticidin s inhibited protein synthesis and caused the degradation of presumptive mrna 1. Blasticidin s hcl solution inhibits peptide bond formation by the ribosome. Blasticidin s hcl solution is a nucleoside antibiotic supplied at 10 mgml in hepes buffer for use as a selection. Blasticidin s hydrochloride was originally isolated by acetone precipitation. First aid measures description of first aid measures skin contact rinse with plenty of water. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. Upon oral application to the mouse, blasticidins and metabolites were excreted in the urine and feces within 24 h. The usual precautions taken when handling chemicals should be observed.
Australian msds statementclassified according to the criteria of worksafe australia. View product specific information, msds, references and buying faq. A method for sensitive determination of blasticidin s bcs in human blood and urine by highperformance liquid chromatography hplc with a spectrophotometric detector has been developed. Blasticidin s hcl supplier cas 3539 tocris bioscience. Blasticidin s hcl is a peptidyl nucleoside produced by several species of streptomyces that was first isolated from s.
Call a poison center or doctorphysician if you feel unwell. If you get a lawn of bacteria on your low salt lb plate instead of individual bacterial colonies, increase the blasticidin concentration to 100. Empirical formula hill notation c 17 h 26 n 8 o 5 hcl. In short, dna of interest is fused to dna encoding a resistance gene, and then is transformed into cells. It works on eukaryotic and prokaryotic cells, and is used to study protein synthesis. Safety data sheet blasticidin s power according to regulation ec no. Blasticidin s hcl solution cas 3539 scbt santa cruz. The working concentration depends on the organism choosen. Blasticidin s, hcl 3539 none none none chemical name korea oel ceilingkorea oel twa korea oel stel blasticidin s, hcl 3539 none none none chemical name taiwan oel twa taiwan oel skin notations, carcinogens taiwan oel stelstaiwan oel ceilings blasticidin s, hcl 3539 none none chemical name singapore oel pel.
Coa, qc data and msds documents are available in one week after order is received. Resistance is conferred by expression of either one of two blasticidin s deaminase genes. For research use only, not for human or veterinary use. Bla477 section 2 hazards identification whmis classification. Blasticidin s hcl is an antibiotic inhibiting protein synthesis. Resistance to blasticidin is conferred by the blasticidin resistance gene from bacillus cereus bsr. Blasticidin s hcl is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic. Upon oral application to the mouse, blasticidin s and metabolites were excreted in the urine and feces within 24 h. Blasticidin s hcl solution is supplied ready to use at 10 mgml in hepes buffer and sterile filtered. Description blasticidin s is an antibiotic that also exhibits antiparasitic activity.
Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet. Blasticidin s hcl is an aminoacyl nucleoside antibiotic isolated from streptomyces griseochromogenes. Carbon oxides, nitrogen oxides, hydrogen chloride 4. Ag scientific s blasticidin s hcl solution is a sterile filterate solution. Download msds pdf search msds currently available for download bioworld offers blasticidin s hydrochloride for your research at low price. Blasticidin is active against both prokaryotic and eukaryotic.
Blasticidin s hydrochloride safety data sheet section 2. The residue level of blasticidin s in rice was below 0. Depending on the bacterial strain used, optimize the blasticidin concentration. Blasticidin s hcl is a nucleoside antibiotic isolated from streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. The facility is registered with the fda as a medical device manufacturer and is certified to iso 485 standards. Blasticidin s hydrochloride is soluble in water or buffers e.
Ag scientifics blasticidin s hcl solution is a sterile filterate solution. Not classified hydrochloric acid, 37% ww 7647 01 0 ld50 oral rat 700 mgkg ld50 dermal rabbit 5010 mgkg. Blasticidin s hydrochloride is a salt of blasticidin s, an amphoteric nucleoside produced by sever al species of streptomyces, first reported in the late 1950s. Blasticidin s is a peptidyl nucleaside antibiotic isolated from the culture broth of streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryotes. Learn more about blasticidin s hcl solid and get the best prices in the life. Blasticidin, from streptomyces griseochromogenes, is an antibiotic commonly used in gene selection.
Quantities above 1 g may be subject to additional shipping fees. This products resides on a fisher scientific gsa or va contract. Supplied as a 10mgml sterile solution in hepes buffer. Blasticidin s, hcl no data available no data available no data available. Product name blasticidin s hcl chemical name synonyms lkt laboratories, inc 545 phalen blvd. Blasticidin s is a nucleoside antibiotic isolated from streptomyces.
Safety data sheet according to federal register vol. View and buy high purity blasticidin s hcl from tocris bioscience. Blasticidin s, hcl, streptomyces griseochromogenes, 10mgml classified as hazardous component as solid state casno 3539 ec number. Blasticidin s hcl stock solution mammalian210 mgml 1. Pdf highperformance liquid chromatographic method for the. If you are viewing this page as a nonregistered user, the price s displayed is list price. To view your gsa or va contract pricing, log in using your account number, or become a registered user by contacting one of our customer service teams.
Rpi blasticidin s hydrochloride solution, 100mg liquid. Medium containing blasticidin can be stored at 4c for up to 2 weeks. For historical reasons only the salt has been routinely available to. Blasticidin s is an antibiotic that is produced by streptomyces griseochromogenes. Safety data sheet creation date 22jul2010 revision date 18jan2018 revision number 3 1. Blasticidin, from streptomyces griseochromogenes, is an antibiotic commonly used. Blasticidin s hydrochloride cas 3539 scbt santa cruz. Blasticidin s hcl, powder thermo fisher scientific. Figure 1 time course of selection with blasticidin. Eye contact rinse cautiously with water for several minutes. It functions by inhibiting peptide bond formation at the ribosome in both eukaryotic and prokaryotic cells. Since the informations vary, it is recommended to determine the.
Blasticidin s hcl is lot qualified by performing a kill curve on blasticidinsensitive and blasticidinresistant mammalian cell lines. Soluble in water, slightly alkaline, or acid solutions. Blasticidin s hydrochloride purity 98%, is in stock. Identification product name blasticidin s hydrochloride cat no. Benzenesulfonic acid an overview sciencedirect topics. Self contained breathing apparatus or nioshmsha approved respirator is recommended. Current shipping out time is about 2 weeks after order is received. If you are viewing this page as a nonregistered user, the prices displayed is list price. For historical reasons only the salt has been routinely available to researchers. Blasticidin s inhibits aflatoxin production in species of aspergillus. However, it seems javascript is either disabled or not supported by your browser. Blasticidinsensitive cells should be killed at all concentrations tested 2. Blasticidin s hydrochloride is used to inhibit protein synthesis in prokaryotic. Javascript must be enabled in order for you to use this site.
New metabolic pathway for converting blasticidin s in aspergillus flavus and inhibitory activity of aflatoxin production by blasticidin s metabolites. Relevant identified uses of the substance or mixture and uses advised against identified uses laboratory reagent. Blasticidine s hydrochloride blasticidin s sigmaaldrich. Blasticidin s hcl b001 blasticidin s b052 blasticidin s hcl solution 10 mgml in 20 mm hepesb006b007 blasticidin s is soluble in water 510 mgml and acetic acid. Pdf highperformance liquid chromatographic method for. Blasticidin s inhibits protein synthesis by inducing deformations in psite trna. Blasticidin s hcl 10 mgml thermo fisher scientific.
Gibco blasticidin s hcl is manufactured at a cgmp compliant facility located in grand island, new york. B4402 blasticidin s hydrochloride references yoshinari t, sakuda s, watanabe m, et al. Information on toxicological effects likely routes of exposure. Blasticidin s, hcl 3539 98100 3539 98100 acute tox.
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